Role of hemagglutinin cleavage and expression of M1 protein in replication of A/WS/33, A/PR/8/34, and WSN influenza viruses in mouse brain.
Identifieur interne : 002134 ( Main/Exploration ); précédent : 002133; suivant : 002135Role of hemagglutinin cleavage and expression of M1 protein in replication of A/WS/33, A/PR/8/34, and WSN influenza viruses in mouse brain.
Auteurs : R W Schlesinger ; G L Bradshaw ; F. Barbone ; M. Reinacher ; R. Rott ; P. HusakSource :
- Journal of Virology [ 0022-538X ] ; 1989.
Abstract
The combined presence of WSN gene segments 6 (neuraminidase), 7 (M1 and M2), and 8 (NS1 and NS2) in reassortants of WSN with A/Aichi/2/68 (H3N2) has been found by others to be necessary for full expression of neurovirulence in mice. We are examining the expression of the analogous three gene segments in brains of mice after intracerebral infection with non-neuroadapted strains A/WS/33 (WS) (from which WSN was derived) and A/PR/8/34 (PR8). Our aim is to determine possible mechanisms by which one or more of the five gene products may restrict replication of these strains in mouse brain cells to a single cycle, yielding noninfectious hemagglutinating particles (incomplete growth cycle). We found that minority subsets of such particles did produce plaques, provided they were activated by trypsin (analogous to other abortive systems producing virions with uncleaved HA), a step obviated for some WSN virions by indirect promotion of hemagglutinin cleavage by the neuraminidase of that strain. The percentage of such potentially infectious virions, relative to total hemagglutinating particles, was significantly lower in WS- or PR8-infected than in WSN-infected brains, suggesting possible defects in synthesis or function of M1 protein in the former. Cells in immunostained sections and appropriate bands in Western blots (immunoblots) of viral proteins electrophoretically separated from lysates of PR8-infected brains reacted with antibody to nucleoprotein but not to M1 protein. Either method revealed the presence of both proteins in WSN-infected brains. In contrast, Western blot analyses of particles concentrated from PR8-, WS-, or WSN-infected brains by hemadsorption, elution, and pelleting did reveal NP and M1 bands with comparable relative peroxidase-antiperoxidase staining intensities. The findings suggest that availability of M1 protein is a factor influencing the extent or rate of assembly of potentially infectious (i.e., trypsin-activated) progeny virions in mouse brains and that in this respect the two non-neurovirulent strains differ from WSN quantitatively rather than qualitatively.
Url:
PubMed: 2648024
PubMed Central: 248424
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000399
- to stream Pmc, to step Curation: 000399
- to stream Pmc, to step Checkpoint: 000D25
- to stream Ncbi, to step Merge: 000C34
- to stream Ncbi, to step Curation: 000C34
- to stream Ncbi, to step Checkpoint: 000C34
- to stream Main, to step Merge: 002236
- to stream Main, to step Curation: 002134
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Role of hemagglutinin cleavage and expression of M1 protein in replication of A/WS/33, A/PR/8/34, and WSN influenza viruses in mouse brain.</title><author><name sortKey="Schlesinger, R W" sort="Schlesinger, R W" uniqKey="Schlesinger R" first="R W" last="Schlesinger">R W Schlesinger</name></author><author><name sortKey="Bradshaw, G L" sort="Bradshaw, G L" uniqKey="Bradshaw G" first="G L" last="Bradshaw">G L Bradshaw</name></author><author><name sortKey="Barbone, F" sort="Barbone, F" uniqKey="Barbone F" first="F" last="Barbone">F. Barbone</name></author><author><name sortKey="Reinacher, M" sort="Reinacher, M" uniqKey="Reinacher M" first="M" last="Reinacher">M. Reinacher</name></author><author><name sortKey="Rott, R" sort="Rott, R" uniqKey="Rott R" first="R" last="Rott">R. Rott</name></author><author><name sortKey="Husak, P" sort="Husak, P" uniqKey="Husak P" first="P" last="Husak">P. Husak</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">2648024</idno><idno type="pmc">248424</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC248424</idno><idno type="RBID">PMC:248424</idno><date when="1989">1989</date><idno type="wicri:Area/Pmc/Corpus">000399</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000399</idno><idno type="wicri:Area/Pmc/Curation">000399</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000399</idno><idno type="wicri:Area/Pmc/Checkpoint">000D25</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000D25</idno><idno type="wicri:Area/Ncbi/Merge">000C34</idno><idno type="wicri:Area/Ncbi/Curation">000C34</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C34</idno><idno type="wicri:doubleKey">0022-538X:1989:Schlesinger R:role:of:hemagglutinin</idno><idno type="wicri:Area/Main/Merge">002236</idno><idno type="wicri:Area/Main/Curation">002134</idno><idno type="wicri:Area/Main/Exploration">002134</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Role of hemagglutinin cleavage and expression of M1 protein in replication of A/WS/33, A/PR/8/34, and WSN influenza viruses in mouse brain.</title><author><name sortKey="Schlesinger, R W" sort="Schlesinger, R W" uniqKey="Schlesinger R" first="R W" last="Schlesinger">R W Schlesinger</name></author><author><name sortKey="Bradshaw, G L" sort="Bradshaw, G L" uniqKey="Bradshaw G" first="G L" last="Bradshaw">G L Bradshaw</name></author><author><name sortKey="Barbone, F" sort="Barbone, F" uniqKey="Barbone F" first="F" last="Barbone">F. Barbone</name></author><author><name sortKey="Reinacher, M" sort="Reinacher, M" uniqKey="Reinacher M" first="M" last="Reinacher">M. Reinacher</name></author><author><name sortKey="Rott, R" sort="Rott, R" uniqKey="Rott R" first="R" last="Rott">R. Rott</name></author><author><name sortKey="Husak, P" sort="Husak, P" uniqKey="Husak P" first="P" last="Husak">P. Husak</name></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="1989">1989</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The combined presence of WSN gene segments 6 (neuraminidase), 7 (M1 and M2), and 8 (NS1 and NS2) in reassortants of WSN with A/Aichi/2/68 (H3N2) has been found by others to be necessary for full expression of neurovirulence in mice. We are examining the expression of the analogous three gene segments in brains of mice after intracerebral infection with non-neuroadapted strains A/WS/33 (WS) (from which WSN was derived) and A/PR/8/34 (PR8). Our aim is to determine possible mechanisms by which one or more of the five gene products may restrict replication of these strains in mouse brain cells to a single cycle, yielding noninfectious hemagglutinating particles (incomplete growth cycle). We found that minority subsets of such particles did produce plaques, provided they were activated by trypsin (analogous to other abortive systems producing virions with uncleaved HA), a step obviated for some WSN virions by indirect promotion of hemagglutinin cleavage by the neuraminidase of that strain. The percentage of such potentially infectious virions, relative to total hemagglutinating particles, was significantly lower in WS- or PR8-infected than in WSN-infected brains, suggesting possible defects in synthesis or function of M1 protein in the former. Cells in immunostained sections and appropriate bands in Western blots (immunoblots) of viral proteins electrophoretically separated from lysates of PR8-infected brains reacted with antibody to nucleoprotein but not to M1 protein. Either method revealed the presence of both proteins in WSN-infected brains. In contrast, Western blot analyses of particles concentrated from PR8-, WS-, or WSN-infected brains by hemadsorption, elution, and pelleting did reveal NP and M1 bands with comparable relative peroxidase-antiperoxidase staining intensities. The findings suggest that availability of M1 protein is a factor influencing the extent or rate of assembly of potentially infectious (i.e., trypsin-activated) progeny virions in mouse brains and that in this respect the two non-neurovirulent strains differ from WSN quantitatively rather than qualitatively.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jvirol00071-0217-a" xlink:role="1699"></graphic></fig><fig id="F2"><graphic xlink:href="jvirol00071-0218-a" xlink:role="1700"></graphic></fig><fig id="F3"><graphic xlink:href="jvirol00071-0219-a" xlink:role="1701"></graphic></fig></sec></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Barbone, F" sort="Barbone, F" uniqKey="Barbone F" first="F" last="Barbone">F. Barbone</name><name sortKey="Bradshaw, G L" sort="Bradshaw, G L" uniqKey="Bradshaw G" first="G L" last="Bradshaw">G L Bradshaw</name><name sortKey="Husak, P" sort="Husak, P" uniqKey="Husak P" first="P" last="Husak">P. Husak</name><name sortKey="Reinacher, M" sort="Reinacher, M" uniqKey="Reinacher M" first="M" last="Reinacher">M. Reinacher</name><name sortKey="Rott, R" sort="Rott, R" uniqKey="Rott R" first="R" last="Rott">R. Rott</name><name sortKey="Schlesinger, R W" sort="Schlesinger, R W" uniqKey="Schlesinger R" first="R W" last="Schlesinger">R W Schlesinger</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002134 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002134 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:248424
|texte= Role of hemagglutinin cleavage and expression of M1 protein in replication of A/WS/33, A/PR/8/34, and WSN influenza viruses in mouse brain.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:2648024" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |